BD PharmingenTM PE Mouse Anti- IRF4

Description

Flow cytometric analysis of IRF4 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC were not stimulated Top Left Plot or stimulated Top Right Plot with phytohemagglutinin PHA; 3 days . C57BL/6 mouse splenic B cells were not stimulated Bottom Left Plot or stimulated Bottom Right Plot with lipopolysaccharide LPS; 2 days . PBMC were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-IRF4 antibody Cat. No. Cat. No. 566646/566649; solid line histograms at 0.25 mug/test. Mouse cells were similarly fixed, permeabilized, and stained using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Histograms showing IRF4 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IRF4 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC were not stimulated Top Left Plot or stimulated Top Right Plot with phytohemagglutinin PHA; 3 days . C57BL/6 mouse splenic B cells were not stimulated Bottom Left Plot or stimulated Bottom Right Plot with lipopolysaccharide LPS; 2 days . PBMC were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-IRF4 antibody Cat. No. Cat. No. 566646/566649; solid line histograms at 0.25 mug/test. Mouse cells were similarly fixed, permeabilized, and stained using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Histograms showing IRF4 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IRF4 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC were not stimulated Top Left Plot or stimulated Top Right Plot with phytohemagglutinin PHA; 3 days . C57BL/6 mouse splenic B cells were not stimulated Bottom Left Plot or stimulated Bottom Right Plot with lipopolysaccharide LPS; 2 days . PBMC were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-IRF4 antibody Cat. No. Cat. No. 566646/566649; solid line histograms at 0.25 mug/test. Mouse cells were similarly fixed, permeabilized, and stained using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Histograms showing IRF4 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto II System. Data shown on this Technical Data Sheet are not lot specific.