BD PharmingenTM PE Mouse anti-Islet-1

Description

Flow cytometric analysis of Islet-1 in mouse pancreatic tumor insulinoma cells . Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed line, Cat. No. 554680 or PE Mouse anti-Islet-1 monoclonal antibody solid line at matched concentrations. This antibody cross-reacts with Islet-2 as well. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Islet-1 in cardiomyocytes derived from human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI were differentiated towards a cardiac lineage Xu C. et al, 2011 , fixed with BD CytofixFixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-Islet-1 antibody solid line at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of H7-derived cardiomyocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of Islet-1 in mouse pancreatic tumor insulinoma cells . Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed line, Cat. No. 554680 or PE Mouse anti-Islet-1 monoclonal antibody solid line at matched concentrations. This antibody cross-reacts with Islet-2 as well. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Flow cytometric analysis of Islet-1 in cardiomyocytes derived from human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI were differentiated towards a cardiac lineage Xu C. et al, 2011 , fixed with BD CytofixFixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-Islet-1 antibody solid line at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of H7-derived cardiomyocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of Islet-1 in mouse pancreatic tumor insulinoma cells . Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed line, Cat. No. 554680 or PE Mouse anti-Islet-1 monoclonal antibody solid line at matched concentrations. This antibody cross-reacts with Islet-2 as well. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Islet-1 in cardiomyocytes derived from human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI were differentiated towards a cardiac lineage Xu C. et al, 2011 , fixed with BD CytofixFixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PE Mouse IgG1, kappa isotype control dashed lines, Cat. No.554680 or PE Mouse Anti-Islet-1 antibody solid line at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of H7-derived cardiomyocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.