BD PharmingenTM PE Mouse Anti-Mouse CD283 (TLR3)

Description

Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD283 TLR3 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were surface stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , Alexa Fluor 700 Mouse Anti-Mouse NK1.1 Cat. No. 560515 , BD Horizon BV510 Rat Anti-Mouse Ly-6G and Ly-6C Cat. No. 563040 , and BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 antibodies. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD283 TLR3 antibody Cat. No. 565984; Right Plot . Two-color contour plots showing the correlated expression CD11c versus CD283 TLR3 or Ig Isotype control staining were derived from Ly-6G and Ly-6C- NK1.1- CD3e- gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.