BD PharmingenTM PE Mouse Anti-Mouse CD289 (TLR9)

Description

Multicolor flow cytometric analysis of TLR9 CD289 expression in mouse splenocytes. Mouse splenic leucocytes were stained with Fixable Viability Stain 450 FVS450; Cat. No. 562247 , fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553087/553088/561877 and APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies, and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or PE Mouse Anti-Mouse TLR9 antibody Cat. No. 565640; solid line histograms . The fluorescence histograms Bottom Plots showing total cellular TLR9 CD289 expression or Ig Isotype control staining for the different leucocyte subsets were derived from CD11c versus CD45R/B220 coexpression-gated events with the forward and side light-scatter SSC-A characteristics of intact FVS450 live cell-discriminated leucocyte subsets Top Plots . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of TLR9 CD289 expression in mouse splenocytes. Mouse splenic leucocytes were stained with Fixable Viability Stain 450 FVS450; Cat. No. 562247 , fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553087/553088/561877 and APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies, and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or PE Mouse Anti-Mouse TLR9 antibody Cat. No. 565640; solid line histograms . The fluorescence histograms Bottom Plots showing total cellular TLR9 CD289 expression or Ig Isotype control staining for the different leucocyte subsets were derived from CD11c versus CD45R/B220 coexpression-gated events with the forward and side light-scatter SSC-A characteristics of intact FVS450 live cell-discriminated leucocyte subsets Top Plots . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of TLR9 CD289 expression in mouse splenocytes. Mouse splenic leucocytes were stained with Fixable Viability Stain 450 FVS450; Cat. No. 562247 , fixed, and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553087/553088/561877 and APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies, and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or PE Mouse Anti-Mouse TLR9 antibody Cat. No. 565640; solid line histograms . The fluorescence histograms Bottom Plots showing total cellular TLR9 CD289 expression or Ig Isotype control staining for the different leucocyte subsets were derived from CD11c versus CD45R/B220 coexpression-gated events with the forward and side light-scatter SSC-A characteristics of intact FVS450 live cell-discriminated leucocyte subsets Top Plots . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.