BD PharmingenTM PE Mouse Anti-Mouse CD366 (TIM-3)

Description

Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with Alexa Fluor 647 Anti-Mouse CD8a antibody Cat. No. 557682 and at 0.25 mug per test either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566346; Right Plot . After staining and washing, the cells were suspended in BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 . A contour plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.