BD PharmingenTM PE Mouse Anti-Mouse CD96 (TACTILE)

Description

Multicolor flow cytometric analysis of CD96 TACTILE expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066; Top Plots , BD Horizon BUV395 Mouse Anti-Mouse CD161b,c NK1.1 antibody Cat. No. 564144; Bottom Plots and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Mouse CD96 TACTILE antibody Cat. No. 572237/572238; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Pseudocolor density plots showing the correlated expression of CD96 TACTILE or Ig Isotype control staining versus CD3e or NK-1.1 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD96 TACTILE expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066; Top Plots , BD Horizon BUV395 Mouse Anti-Mouse CD161b,c NK1.1 antibody Cat. No. 564144; Bottom Plots and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Mouse CD96 TACTILE antibody Cat. No. 572237/572238; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Pseudocolor density plots showing the correlated expression of CD96 TACTILE or Ig Isotype control staining versus CD3e or NK-1.1 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD96 TACTILE expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066; Top Plots , BD Horizon BUV395 Mouse Anti-Mouse CD161b,c NK1.1 antibody Cat. No. 564144; Bottom Plots and with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; Left Plots or PE Mouse Anti-Mouse CD96 TACTILE antibody Cat. No. 572237/572238; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Pseudocolor density plots showing the correlated expression of CD96 TACTILE or Ig Isotype control staining versus CD3e or NK-1.1 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.