BD PharmingenTM PE Mouse Anti-Mouse H-2Kb/SIINFEKL

Description

Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leucocytes were either not pulsed Left Panel or were pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; solid line histograms at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with prior SIINFEKL peptide treatment. Mouse splenic leucocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2Kb/SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leucocytes were either not pulsed Left Panel or were pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; solid line histograms at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with prior SIINFEKL peptide treatment. Mouse splenic leucocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2Kb/SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leucocytes were either not pulsed Left Panel or were pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; solid line histograms at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leucocytes with prior SIINFEKL peptide treatment. Mouse splenic leucocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or with PE Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 569791/569792; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2Kb/SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.