BD PharmingenTM PE Mouse anti-Mouse IL-17F

Description

Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of mouse IL-17F expression in resting or stimulated mouse EL4 thymoma cells or in polarized mouse Th17 cells. Mouse EL4 thymoma cells were either unstimulated or stimulated with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139 and Ionomycin Sigma; I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. Th17 cells were generated from Th17-polarizing mouse spleen cell cultures and were stimulated with PMA and Ionomycin with BD GolgiStop Protein Transport Inhibitor. The EL4 and Th17 cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The unstimulated Left Panel and stimulated Middle Panel EL4 cells were stained with PE Mouse Anti-Mouse IL-17F Cat. No. 561627 . To validate O79-289 antibody use for staining IL-17F in Th17 cells generated from primary mouse T cells, the fixed and permeabilized Th17-polarized cells were stained with purified O79-289 Mouse Anti-Mouse IL-17F antibody followed by PE Goat Anti-Mouse Ig Cat. No. 550589 and with Alexa Fluor 488 Rat Anti-Mouse IL-17A antibody Cat. No. 560220 Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus cellular autofluorescence measured in the FL4 channel for EL4 cells or versus IL-17A for Th17 cells were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System.