BD PharmingenTM PE Mouse Anti-Mouse IL-27 p28 (IL-30)

Description

Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-27 p28 IL-30 expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-gamma Cat. No. 554587; 24 h; Left Plot followed by lipopolysaccharide LPS, Sigma L3137, 1 mug/ml; additional 24 h . BD GolgiStop Cat. No. 554724 was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control Right Plot . BD Horizon Fixable Viability Stain 510 FVS510; Cat. No. 564406 was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554722 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dashed line histogram or PE Mouse Anti-Mouse IL-27 p28 IL-30 antibody Cat. No. 568408/568409; solid line histogram at 0.5 mug/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 IL-30 expression or Ig Isotype control staining by RAW264.7 cells treated with Left Plot or without Right Plot IFN-gamma and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.