
560277 ·
BDB Bioscience · Cat: 560277
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometry analysis of PE anti Nanog on mouse embryonic stem ES cells. Mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Nanog solid line or PE Mouse IgG1,k Isotype Control Cat. No. 554680, dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometry analysis of PE anti Nanog on mouse embryonic stem ES cells. Mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Nanog solid line or PE Mouse IgG1,k Isotype Control Cat. No. 554680, dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Flow cytometry analysis of PE anti Nanog on mouse embryonic stem ES cells. Mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Nanog solid line or PE Mouse IgG1,k Isotype Control Cat. No. 554680, dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system.