BD PharmingenTM PE Mouse Anti-Mouse Qa-1(b)

Description

Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Qa-1 b expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J Qa-1 a ; Left Panel or C57BL/6 Qa-1 b ; Right Panel mouse were stimulated for 3 days in culture with lipopolysaccharide LPS . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histograms or PE Mouse Anti-Mouse Qa-1 b antibody Cat. No. 566640/566641; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing Qa-1 b expression or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative , activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.