BD PharmingenTM PE Mouse anti-Mouse RORgammat

Description

Multicolor flow cytometric analysis of mouse RORgammat expression in thymocytes and Th17 polarized cells. BALB/c mouse thymocytes Panel 1 or Th17 polarized cells Panels 2 and 3 were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Mouse Anti-Mouse RORgammat antibody Cat. No. 562607 or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648 . The thymocytes were counterstained with APC Rat Anti-Mouse CD4 Cat. No. 553051 and PerCP-Cy5.5 Rat Anti-Mouse CD8a Cat. No. 551162 antibodies. The Th17-polarized cells were counterstained with APC-Cy7 Hamster Anti-Mouse CD3e Cat. No. 557596 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , FITC Rat Anti-Mouse IFN-gamma Cat. No. 554411 and PerCP-Cy5.5 Rat Anti-Mouse IL-17A Cat. No. 560666 antibodies. Panel 1: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in CD4+CD8- single-positive dotted line or CD4+CD8+ double-positive solid line thymocytes. Panel 2: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in Th17-polarized IL-17A-IFN-gamma+ dotted line or IL-17A+IFN-gamma- solid line cells. Panel 3: Two-color contour plots show the correlated expression patterns of IgG2a Control Left or ROR gamma t Right staining versus IL-17A in Th17-polarized CD3+CD4+ cells. The flow cytometric data were derived from viable cell-discriminated events with the forward and side light-scatter characteristics of intact single cells using a BD LSRFortessa Cytometer System. Multicolor flow cytometric analysis of mouse RORgammat expression in thymocytes and Th17 polarized cells. BALB/c mouse thymocytes Panel 1 or Th17 polarized cells Panels 2 and 3 were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Mouse Anti-Mouse RORgammat antibody Cat. No. 562607 or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648 . The thymocytes were counterstained with APC Rat Anti-Mouse CD4 Cat. No. 553051 and PerCP-Cy5.5 Rat Anti-Mouse CD8a Cat. No. 551162 antibodies. The Th17-polarized cells were counterstained with APC-Cy7 Hamster Anti-Mouse CD3e Cat. No. 557596 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , FITC Rat Anti-Mouse IFN-gamma Cat. No. 554411 and PerCP-Cy5.5 Rat Anti-Mouse IL-17A Cat. No. 560666 antibodies. Panel 1: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in CD4+CD8- single-positive dotted line or CD4+CD8+ double-positive solid line thymocytes. Panel 2: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in Th17-polarized IL-17A-IFN-gamma+ dotted line or IL-17A+IFN-gamma- solid line cells. Panel 3: Two-color contour plots show the correlated expression patterns of IgG2a Control Left or ROR gamma t Right staining versus IL-17A in Th17-polarized CD3+CD4+ cells. The flow cytometric data were derived from viable cell-discriminated events with the forward and side light-scatter characteristics of intact single cells using a BD LSRFortessa Cytometer System. Show More Multicolor flow cytometric analysis of mouse RORgammat expression in thymocytes and Th17 polarized cells. BALB/c mouse thymocytes Panel 1 or Th17 polarized cells Panels 2 and 3 were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Mouse Anti-Mouse RORgammat antibody Cat. No. 562607 or PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648 . The thymocytes were counterstained with APC Rat Anti-Mouse CD4 Cat. No. 553051 and PerCP-Cy5.5 Rat Anti-Mouse CD8a Cat. No. 551162 antibodies. The Th17-polarized cells were counterstained with APC-Cy7 Hamster Anti-Mouse CD3e Cat. No. 557596 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , FITC Rat Anti-Mouse IFN-gamma Cat. No. 554411 and PerCP-Cy5.5 Rat Anti-Mouse IL-17A Cat. No. 560666 antibodies. Panel 1: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in CD4+CD8- single-positive dotted line or CD4+CD8+ double-positive solid line thymocytes. Panel 2: The overlapping histograms show the levels of IgG2a Control Left and RORgammat Right staining in Th17-polarized IL-17A-IFN-gamma+ dotted line or IL-17A+IFN-gamma- solid line cells. Panel 3: Two-color contour plots show the correlated expression patterns of IgG2a Control Left or ROR gamma t Right staining versus IL-17A in Th17-polarized CD3+CD4+ cells. The flow cytometric data were derived from viable cell-discriminated events with the forward and side light-scatter characteristics of intact single cells using a BD LSRFortessa Cytometer System.