BD PharmingenTM PE Mouse Anti-Mouse TCR Vdelta6B

Description

Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vdelta6B expression on Mouse intraepithelial lymphocytes IELs . C57BL/6 Mouse IELs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vdelta6B antibody Cat. No. 570738/570739; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vdelta6B or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3-positive IELs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.