BD PharmingenTM PE Mouse Anti-Mouse TCR Vgamma6

Description

Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , BD Horizon BV421 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 562892 , and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570216/570217; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus gammadelta T-Cell Receptor was derived from gated events with the forward and side light-scatter characteristics of viable CD3e-positive cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.