BD PharmingenTM PE Mouse Anti-Mouse TCR Vgamma7

Description

Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vgamma7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD OptiBuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 antibodies and with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; Left Plot or PE Mouse Anti-Mouse TCR Vgamma7 antibody Cat. No. 569154/569155; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma7 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.