BD PharmingenTM PE Mouse Anti-Myosin Heavy Chain

Description

Analysis of Myosin Heavy Chain in human embryonic stem cell hESC -derived cardiomyocytes. hESC- derived cardiomyocytes Evans laboratory, UC San Diego were disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC and Alexa Fluor 647 Mouse Anti-Cardiac Troponin I cTnI Cat. No. 554409 . The cells were first gated on light scatter properties and then analyzed for MHC and cTnI expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Analysis of Myosin Heavy Chain in differentiated C2C12 mouse myoblast cell line. C2C12 cells ATCC: CRL-1772 that were differentiated for 3 days and then disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC . The cells were first gated on light scatter properties and then analyzed for MHC expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Analysis of Myosin Heavy Chain in human embryonic stem cell hESC -derived cardiomyocytes. hESC- derived cardiomyocytes Evans laboratory, UC San Diego were disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC and Alexa Fluor 647 Mouse Anti-Cardiac Troponin I cTnI Cat. No. 554409 . The cells were first gated on light scatter properties and then analyzed for MHC and cTnI expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Show More Analysis of Myosin Heavy Chain in differentiated C2C12 mouse myoblast cell line. C2C12 cells ATCC: CRL-1772 that were differentiated for 3 days and then disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC . The cells were first gated on light scatter properties and then analyzed for MHC expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Show More Analysis of Myosin Heavy Chain in human embryonic stem cell hESC -derived cardiomyocytes. hESC- derived cardiomyocytes Evans laboratory, UC San Diego were disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC and Alexa Fluor 647 Mouse Anti-Cardiac Troponin I cTnI Cat. No. 554409 . The cells were first gated on light scatter properties and then analyzed for MHC and cTnI expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Analysis of Myosin Heavy Chain in differentiated C2C12 mouse myoblast cell line. C2C12 cells ATCC: CRL-1772 that were differentiated for 3 days and then disassociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 , and stained with PE Mouse Anti-Myosin Heavy Chain MHC . The cells were first gated on light scatter properties and then analyzed for MHC expression. Flow cytometry was performed on a BD LSRFortessa flow cytometry system.