BD PharmingenTM PE Mouse anti-Nestin

Description

Analysis of Nestin expression in rat glioma. C6 cells ATCC, Cat. No. CCL-107 were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III. Analysis of Nestin expression in human Neural Stem Cells NSC . NSC were derived from H9 human embryonic stem cells WiCell, Wisconsin and grown for 8 passages, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III. Analysis of Nestin expression in rat glioma. C6 cells ATCC, Cat. No. CCL-107 were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III. Show More Analysis of Nestin expression in human Neural Stem Cells NSC . NSC were derived from H9 human embryonic stem cells WiCell, Wisconsin and grown for 8 passages, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III. Show More Analysis of Nestin expression in rat glioma. C6 cells ATCC, Cat. No. CCL-107 were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III. Analysis of Nestin expression in human Neural Stem Cells NSC . NSC were derived from H9 human embryonic stem cells WiCell, Wisconsin and grown for 8 passages, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either PE Mouse anti-Nestin solid line or PE Mouse IgG1, kappa Isotype Control Clone MOPC-21, Cat. No. 554680, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This antibody conjugate is also compatible with BD Phosflow Perm Buffers II and III.