
564730 ·
BDB Bioscience · Cat: 564730
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293 - F cells Life Technologies R790-07 were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 . Fixed cells were then stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058, dashed line or PE Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293 - F cells Life Technologies R790-07 were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 . Fixed cells were then stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058, dashed line or PE Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293 - F cells Life Technologies R790-07 were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 . Fixed cells were then stained with either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058, dashed line or PE Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System.