
560186 ·
BDB Bioscience · Cat: 560186
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Analysis of Oct3/4 in human embryonal carcinoma cells. NCCIT cells ATCC CRL-2073 were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Oct3/4 shaded histogram or PE Mouse IgG1, kappa Isotype Control open histogram . Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Oct3/4 in human embryonal carcinoma cells. NCCIT cells ATCC CRL-2073 were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Oct3/4 shaded histogram or PE Mouse IgG1, kappa Isotype Control open histogram . Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of Oct3/4 in human embryonal carcinoma cells. NCCIT cells ATCC CRL-2073 were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either PE Mouse anti-Oct3/4 shaded histogram or PE Mouse IgG1, kappa Isotype Control open histogram . Flow cytometry was performed on a BD LSR II flow cytometry system.