BD PharmingenTM PE Mouse Anti-Pig CD4a

Description

CD4 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 74-12-4 and FITC-conjugated anti-pig CD3a BB23-8E6-8C8 Cat. no. 559582 monoclonal antibodies. Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. CD4 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 74-12-4 and FITC-conjugated anti-pig CD3a BB23-8E6-8C8 Cat. no. 559582 monoclonal antibodies. Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More CD4 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 74-12-4 and FITC-conjugated anti-pig CD3a BB23-8E6-8C8 Cat. no. 559582 monoclonal antibodies. Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.