BD PharmingenTM PE Mouse Anti-PLZF

Description

Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes. Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-PLZF antibody Cat. No. 564850; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 . The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash Buffer and stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-PLZF antibody Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes. Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-PLZF antibody Cat. No. 564850; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 . The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash Buffer and stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-PLZF antibody Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes. Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-PLZF antibody Cat. No. 564850; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 . The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash Buffer and stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-PLZF antibody Right Plot . The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System.