BD PharmingenTM PE Mouse Anti-Rat CD4

Description

Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD4 expression on Lewis rat splenocytes. Splenocytes from a Lewis rat were stained with APC Mouse Anti-Rat CD3 antibody Cat. No. 557030 and with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Rat CD4 antibody Cat. No. 572406/572407; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Lewis rat splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.