BD PharmingenTM PE Mouse Anti-TCF-7/TCF-1

Description

Two-color flow cytometric analysis of TCF-7/TCF-1 expression. Left Panel - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE-Cy7 Mouse Anti-Human CD4 antibody Cat. No. 557852 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 564217; Right Plot . Right Panel - Mouse splenic leucocytes SPL were similarly fixed and permeabilized. The cells were then stained with PE-Cy7 Rat Anti-Mouse CD4 antibody Cat. No. 552775 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus TCF-7/TCF-1 for gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of TCF-7/TCF-1 expression. Left Panel - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE-Cy7 Mouse Anti-Human CD4 antibody Cat. No. 557852 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 564217; Right Plot . Right Panel - Mouse splenic leucocytes SPL were similarly fixed and permeabilized. The cells were then stained with PE-Cy7 Rat Anti-Mouse CD4 antibody Cat. No. 552775 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus TCF-7/TCF-1 for gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of TCF-7/TCF-1 expression. Left Panel - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE-Cy7 Mouse Anti-Human CD4 antibody Cat. No. 557852 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 564217; Right Plot . Right Panel - Mouse splenic leucocytes SPL were similarly fixed and permeabilized. The cells were then stained with PE-Cy7 Rat Anti-Mouse CD4 antibody Cat. No. 552775 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-TCF-7/TCF-1 antibody Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus TCF-7/TCF-1 for gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.