BD PharmingenTM PE Mouse IgG2a, kappa Isotype Control

Description

Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel . Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel . Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel . Show More Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel . Show More Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel . Expression of human MIP-1alpha by stimulated CD14+ human lymphocytes. Human PBMC were stimulated for 6 hours with LPS 100 ng/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1alpha Cat. No. 554730, top panel , or PE-mouse IgG2akappa Cat. No. 559319; middle panel , by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls bottom panel .