BD PharmingenTM PE Mouse IgG2b, kappa Isotype Control

Description

Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Show More Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Show More Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS 1.0 mug/ml final concentration in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with 0.25 mug of FITC-mouse anti-human CD14 monoclonal antibody FITC-M5E2, Cat. No. 555397 , fixed, permeabilized, and subsequently stained with either 0.125 mug of PE-anti-human IL-8 Cat. No. 554720; left panel , or 0.125 mug of PE-mouse IgG2b Cat. No. 555058; middle panel , following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel .