BD PharmingenTM PE Rabbit Anti-Active Caspase-3

Description

Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Human Jurkat cells. Cells from the Human Jurkat T-cell leukemia, ATCC TIB-152 cell line were left untreated Left histogram or treated with 12 muM of Camptothecin for 6 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Mouse thymocytes. C57BL/6 Mouse thymocytes were left untreated Left histogram or treated with 1 muM of Dexamethasone for 5 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Human Jurkat cells. Cells from the Human Jurkat T-cell leukemia, ATCC TIB-152 cell line were left untreated Left histogram or treated with 12 muM of Camptothecin for 6 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Mouse thymocytes. C57BL/6 Mouse thymocytes were left untreated Left histogram or treated with 1 muM of Dexamethasone for 5 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Human Jurkat cells. Cells from the Human Jurkat T-cell leukemia, ATCC TIB-152 cell line were left untreated Left histogram or treated with 12 muM of Camptothecin for 6 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Active Caspase-3 expression by Apoptotic and Non-apoptotic Mouse thymocytes. C57BL/6 Mouse thymocytes were left untreated Left histogram or treated with 1 muM of Dexamethasone for 5 hours to induce apoptosis Right histogram . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained in BD Perm/Wash Buffer with PE Rabbit Anti-Active Caspase-3 antibody Cat. No. 570183/570184 . The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scatter characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.