BD PharmingenTM PE Rat Anti-beta-galactosidase

Description

Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of beta-galactosidase expression in Escherichia coli strain K12 beta-galactosidase-transfected cells. Non-transfected cells Left Plot or beta-galactosidase-transfected cells Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; dashed line histogram or PE Rat Anti-beta-galactosidase antibody Cat. No. 570542/570543; solid line histogram at 0.25 mug/test. The fluorescence histogram showing beta-galactosidase expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.