BD PharmingenTM PE Rat Anti-Blimp-1

Description

Flow Cytometric Analysis of Blimp-1 Expression Left Panel - Human Peripheral Blood Lymphocytes. Human PBMCs were stained in BD Horizon Brilliant Stain Buffer Cat. No. 563794 with BV786 Mouse Anti-Human CD27 Cat No. 563327 and BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with PE Rat Anti-Blimp-1 antibody Cat. No. 564702 . The two-color contour plot shows the coexpression of CD19 vs CD27 for gated events with the forward and side light-scatter characteristics of live lymphocytes. Histograms showing Blimp-1 expression for B cell subsets were derived from gated events as indicated. Flow cytometric analysis was performed with a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - In Vitro-Differentiated Human Plasma Cells. CD19+CD27+ cells were sorted from PBMCs using the BD IMag Human B Lymphocyte Enrichment Set Cat No 558007 followed by FACS sorting with a BD FACSAria III. Sorted cells were cultured 10 days per Jourdan M, et al 2009 for differentiation of plasma cells. Cells were initially stained with BD Horizon Fixable Viability Stain 510 FVS 510; Cat. No. 564406 for dead cell exclusion. Cells were surface stained with APC Anti-Human CD38 Cat No. 555462 and BV421 Anti-Human CD138 Cat No. 562935 antibodies. Cells were then fixed and permeabilized with the Transcription Factor Buffer Set and stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 554689 or PE Rat Anti-Blimp-1 antibody. The two-color contour plot shows the coexpression of CD38 vs CD138 by live cells identified as FVS 510 negative. Plasma cells were identified as CD38 + CD138 + cells and gated for the generation of the histograms showing Blimp-1 expression solid line versus Isotype Control staining dashed line . Flow cytometric analysis was performed with a BD FACSCanto II Flow Cytometer System. Flow Cytometric Analysis of Blimp-1 Expression Left Panel - Human Peripheral Blood Lymphocytes. Human PBMCs were stained in BD Horizon Brilliant Stain Buffer Cat. No. 563794 with BV786 Mouse Anti-Human CD27 Cat No. 563327 and BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with PE Rat Anti-Blimp-1 antibody Cat. No. 564702 . The two-color contour plot shows the coexpression of CD19 vs CD27 for gated events with the forward and side light-scatter characteristics of live lymphocytes. Histograms showing Blimp-1 expression for B cell subsets were derived from gated events as indicated. Flow cytometric analysis was performed with a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - In Vitro-Differentiated Human Plasma Cells. CD19+CD27+ cells were sorted from PBMCs using the BD IMag Human B Lymphocyte Enrichment Set Cat No 558007 followed by FACS sorting with a BD FACSAria III. Sorted cells were cultured 10 days per Jourdan M, et al 2009 for differentiation of plasma cells. Cells were initially stained with BD Horizon Fixable Viability Stain 510 FVS 510; Cat. No. 564406 for dead cell exclusion. Cells were surface stained with APC Anti-Human CD38 Cat No. 555462 and BV421 Anti-Human CD138 Cat No. 562935 antibodies. Cells were then fixed and permeabilized with the Transcription Factor Buffer Set and stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 554689 or PE Rat Anti-Blimp-1 antibody. The two-color contour plot shows the coexpression of CD38 vs CD138 by live cells identified as FVS 510 negative. Plasma cells were identified as CD38 + CD138 + cells and gated for the generation of the histograms showing Blimp-1 expression solid line versus Isotype Control staining dashed line . Flow cytometric analysis was performed with a BD FACSCanto II Flow Cytometer System. Show More Flow Cytometric Analysis of Blimp-1 Expression Left Panel - Human Peripheral Blood Lymphocytes. Human PBMCs were stained in BD Horizon Brilliant Stain Buffer Cat. No. 563794 with BV786 Mouse Anti-Human CD27 Cat No. 563327 and BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with PE Rat Anti-Blimp-1 antibody Cat. No. 564702 . The two-color contour plot shows the coexpression of CD19 vs CD27 for gated events with the forward and side light-scatter characteristics of live lymphocytes. Histograms showing Blimp-1 expression for B cell subsets were derived from gated events as indicated. Flow cytometric analysis was performed with a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - In Vitro-Differentiated Human Plasma Cells. CD19+CD27+ cells were sorted from PBMCs using the BD IMag Human B Lymphocyte Enrichment Set Cat No 558007 followed by FACS sorting with a BD FACSAria III. Sorted cells were cultured 10 days per Jourdan M, et al 2009 for differentiation of plasma cells. Cells were initially stained with BD Horizon Fixable Viability Stain 510 FVS 510; Cat. No. 564406 for dead cell exclusion. Cells were surface stained with APC Anti-Human CD38 Cat No. 555462 and BV421 Anti-Human CD138 Cat No. 562935 antibodies. Cells were then fixed and permeabilized with the Transcription Factor Buffer Set and stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 554689 or PE Rat Anti-Blimp-1 antibody. The two-color contour plot shows the coexpression of CD38 vs CD138 by live cells identified as FVS 510 negative. Plasma cells were identified as CD38 + CD138 + cells and gated for the generation of the histograms showing Blimp-1 expression solid line versus Isotype Control staining dashed line . Flow cytometric analysis was performed with a BD FACSCanto II Flow Cytometer System.