BD PharmingenTM PE Rat Anti-Eos

Description

Flow cytometric analysis of Eos expression in mouse splenic regulatory T lymphocytes. Spleen cells from C57BL/6 mice were stained intracellularly with Alexa Fluor 647 Rat Anti-mouse Foxp3 Cat. No. 560401 or 560402 , Alexa Fluor 488 Rat Anti-Mouse CD4 Cat. No. 557667 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact leukocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus Foxp3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Eos expression in human regulatory T lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with Alexa Fluor 647 Mouse anti-Human FoxP3 Cat. No. 560045 or 560889 , BV421 Mouse Anti-Human CD4 Cat. No. 562424 or 562425 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus FoxP3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Eos expression in mouse splenic regulatory T lymphocytes. Spleen cells from C57BL/6 mice were stained intracellularly with Alexa Fluor 647 Rat Anti-mouse Foxp3 Cat. No. 560401 or 560402 , Alexa Fluor 488 Rat Anti-Mouse CD4 Cat. No. 557667 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact leukocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus Foxp3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Eos expression in human regulatory T lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with Alexa Fluor 647 Mouse anti-Human FoxP3 Cat. No. 560045 or 560889 , BV421 Mouse Anti-Human CD4 Cat. No. 562424 or 562425 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus FoxP3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Eos expression in mouse splenic regulatory T lymphocytes. Spleen cells from C57BL/6 mice were stained intracellularly with Alexa Fluor 647 Rat Anti-mouse Foxp3 Cat. No. 560401 or 560402 , Alexa Fluor 488 Rat Anti-Mouse CD4 Cat. No. 557667 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact leukocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus Foxp3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Eos expression in human regulatory T lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with Alexa Fluor 647 Mouse anti-Human FoxP3 Cat. No. 560045 or 560889 , BV421 Mouse Anti-Human CD4 Cat. No. 562424 or 562425 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Top Row or PE Rat Anti-Eos Cat. No. 566747; Bottom Row at 0.06ug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 or 562574 . Left Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus CD4 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Right Column: Two-parameter flow cytometric dot plots showing the correlated expression of Eos or Ig isotype control staining versus FoxP3 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.