BD PharmingenTM PE Rat Anti-Human Clec9A (CD370)

Description

Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Show More Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Show More Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Human Clec9A CD370 antibody Cat. No. 563488; Right Panel . The cells were also stained with FITC Mouse Anti-Human HLA-DR Cat No. 555811 , BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 and a Lineage cocktail comprised of BD Horizon V450-conjugated Mouse Anti-Human CD3 Cat. No. 560366 , Mouse Anti-Human CD14 Cat. No. 560350 , Mouse Anti-Human CD19 Cat. No. 560354 and Mouse Anti-Human CD56 Cat. No. 560361 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A CD370 or Ig Isotype control staining versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System.