BD PharmingenTM PE Rat Anti-Human IL-2

Description

Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for IL-2 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus macaque PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PE Rat IgG2a, kappa isotype control Cat. No. 559317; left panel or with the PE Rat Anti-Human IL-2 antibody Cat. No. 560709/554656/554657; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.