BD PharmingenTM PE Rat Anti-Mouse Active Integrin beta7

Description

Two-color flow cytometric analysis of Active Integrin beta7 expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed in Hanks Balanced Salt Solution HBSS , and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained in HBSS Top Plots or HBSS + Mn2+ Bottom Plots with BD OptiBuild BV421 Integrin beta7 chain antibody Cat No. 743787 , and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930/554689; Left Plots or PE Rat Anti-Mouse Active Integrin beta7 antibody Cat. No. 571202/571203; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Active Integrin beta7 or Ig Isotype control staining versus Integrin beta7 chain were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Active Integrin beta7 expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed in Hanks Balanced Salt Solution HBSS , and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained in HBSS Top Plots or HBSS + Mn2+ Bottom Plots with BD OptiBuild BV421 Integrin beta7 chain antibody Cat No. 743787 , and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930/554689; Left Plots or PE Rat Anti-Mouse Active Integrin beta7 antibody Cat. No. 571202/571203; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Active Integrin beta7 or Ig Isotype control staining versus Integrin beta7 chain were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Active Integrin beta7 expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed in Hanks Balanced Salt Solution HBSS , and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained in HBSS Top Plots or HBSS + Mn2+ Bottom Plots with BD OptiBuild BV421 Integrin beta7 chain antibody Cat No. 743787 , and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930/554689; Left Plots or PE Rat Anti-Mouse Active Integrin beta7 antibody Cat. No. 571202/571203; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Active Integrin beta7 or Ig Isotype control staining versus Integrin beta7 chain were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.