BD PharmingenTM PE Rat Anti-Mouse C3aR

Description

Flow cytometric analysis of C3aR expression on mouse Peritoneal Exudate Cells PECs . C57BL/6 mouse PECs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat No. 565853 and with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; Left Plot or PE Rat Anti-Mouse C3aR antibody Cat No. 568276; Right Plot at 0.5 mug/test. The two-color flow cytometric contour plot showing the corelated expression of C3aR or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of C3aR expression on mouse J774A.1 cells. Cells from the J774A.1 Mouse macrophage, ATCC TIB-67 cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; dashed line histogram or PE Rat Anti-Mouse C3aR antibody Cat No. 568276: solid line histogram at 0.5 mug/test. The fluorescence histogram showing C3aR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable mouse J774A.1 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of C3aR expression on mouse Peritoneal Exudate Cells PECs . C57BL/6 mouse PECs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat No. 565853 and with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; Left Plot or PE Rat Anti-Mouse C3aR antibody Cat No. 568276; Right Plot at 0.5 mug/test. The two-color flow cytometric contour plot showing the corelated expression of C3aR or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of C3aR expression on mouse J774A.1 cells. Cells from the J774A.1 Mouse macrophage, ATCC TIB-67 cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; dashed line histogram or PE Rat Anti-Mouse C3aR antibody Cat No. 568276: solid line histogram at 0.5 mug/test. The fluorescence histogram showing C3aR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable mouse J774A.1 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of C3aR expression on mouse Peritoneal Exudate Cells PECs . C57BL/6 mouse PECs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat No. 565853 and with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; Left Plot or PE Rat Anti-Mouse C3aR antibody Cat No. 568276; Right Plot at 0.5 mug/test. The two-color flow cytometric contour plot showing the corelated expression of C3aR or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of C3aR expression on mouse J774A.1 cells. Cells from the J774A.1 Mouse macrophage, ATCC TIB-67 cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either PE Rat IgG2a, kappa Isotype Control Cat No. 553930; dashed line histogram or PE Rat Anti-Mouse C3aR antibody Cat No. 568276: solid line histogram at 0.5 mug/test. The fluorescence histogram showing C3aR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable mouse J774A.1 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.