BD PharmingenTM PE Rat Anti-Mouse CD117 (c-kit)

Description

Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R Cat. No. 553092/561880 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471; Right Plot at 0.06 mug/test. A pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System equipped with a Blue Laser and FlowJo software. Data shown in this Technical Data Sheet are not lot specific.