BD PharmingenTM PE Rat Anti-Mouse CD124

Description

Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel. Show More Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel. Show More Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with PE Rat Anti-Mouse CD124 Cat. No 561695; 0.25 mug/10e6 cells and FITC Rat Anti-Mouse CD45R/B220 0.06 mug; Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the PE-mIL4R-M1 antibody right panel is compared to staining derived with a PE Rat IgG2a, kappa Isotype Control antibody 0.25 mug, Cat. No. 553930 that is shown in left panel.