BD PharmingenTM PE Rat Anti-Mouse CD140b (PDGFRbeta)

Description

Flow cytometric analysis of CD140b expression on Mouse splenic leucocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenic leucocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD140b expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of the correlated expression of CD140a and CD140b on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 and BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 Left Plot or with PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797 and BD Horizon BV421 Rat Anti-Mouse CD140a antibody Cat. No. 562774 Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD140b expression on Mouse splenic leucocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenic leucocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD140b expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of the correlated expression of CD140a and CD140b on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 and BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 Left Plot or with PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797 and BD Horizon BV421 Rat Anti-Mouse CD140a antibody Cat. No. 562774 Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD140b expression on Mouse splenic leucocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenic leucocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD140b expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of the correlated expression of CD140a and CD140b on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 and BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 Left Plot or with PE Rat Anti-Mouse CD140b antibody Cat. No. 569796/569797 and BD Horizon BV421 Rat Anti-Mouse CD140a antibody Cat. No. 562774 Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.