BD PharmingenTM PE Rat Anti-Mouse CD169 (Siglec-1)

Description

Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD169 Siglec -1 expression on Mouse myeloid cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; Left Plot or PE Rat Anti-Mouse CD169 Siglec-1 antibody Cat. No. 570770/570848; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 Siglec-1 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.