BD PharmingenTM PE Rat Anti-Mouse CD186 (CXCR6)

Description

Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD186 CXCR6 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 55314 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD186 CXCR6 antibody Cat. No. 571900/571901; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD186 CXCR6 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.