BD PharmingenTM PE Rat Anti-Mouse CD205 (DEC-205)

Description

Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. Washed BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plots or PE Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567207; Right Plots at 0.25 mug/test. Bivariate pseudocolor plots showing the correlated expression of Ly-6G and Ly-6C versus CD205 DEC-205 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed on the same stained cell samples using a BD LSRFortessa X-20 Cell Analyzer System equipped with both a Yellow-Green Laser Top Plots and a Blue Laser Bottom Plots and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. Washed BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plots or PE Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567207; Right Plots at 0.25 mug/test. Bivariate pseudocolor plots showing the correlated expression of Ly-6G and Ly-6C versus CD205 DEC-205 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed on the same stained cell samples using a BD LSRFortessa X-20 Cell Analyzer System equipped with both a Yellow-Green Laser Top Plots and a Blue Laser Bottom Plots and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. Washed BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 553129/561083 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plots or PE Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567207; Right Plots at 0.25 mug/test. Bivariate pseudocolor plots showing the correlated expression of Ly-6G and Ly-6C versus CD205 DEC-205 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed on the same stained cell samples using a BD LSRFortessa X-20 Cell Analyzer System equipped with both a Yellow-Green Laser Top Plots and a Blue Laser Bottom Plots and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.