BD PharmingenTM PE Rat Anti-Mouse CD206

Description

Analysis of CD206 Expression by Thioglycolate-elicited Peritoneal Exudate Cells Thio PECs Surface CD206 Expression - Panel 1: Thio PECs were stained with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 or PE Rat Anti-Mouse CD206 antibody Cat. No. 568273 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI negative cells. Surface and Intracellular CD206 Expression - Panels 2 and 3: Thio PECs were either fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 Panel 2 or alternatively were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 Panel 3 and were stained as previously described at 0.5 mug/test and indicated. The bivariate plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments. Analysis of CD206 Expression by Thioglycolate-elicited Peritoneal Exudate Cells Thio PECs Surface CD206 Expression - Panel 1: Thio PECs were stained with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 or PE Rat Anti-Mouse CD206 antibody Cat. No. 568273 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI negative cells. Surface and Intracellular CD206 Expression - Panels 2 and 3: Thio PECs were either fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 Panel 2 or alternatively were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 Panel 3 and were stained as previously described at 0.5 mug/test and indicated. The bivariate plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments. Show More Analysis of CD206 Expression by Thioglycolate-elicited Peritoneal Exudate Cells Thio PECs Surface CD206 Expression - Panel 1: Thio PECs were stained with Alexa Fluor 647 Rat Anti-Mouse CD107b antibody Cat. No. 564843 and with either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 or PE Rat Anti-Mouse CD206 antibody Cat. No. 568273 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI negative cells. Surface and Intracellular CD206 Expression - Panels 2 and 3: Thio PECs were either fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 Panel 2 or alternatively were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 Panel 3 and were stained as previously described at 0.5 mug/test and indicated. The bivariate plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments.