BD PharmingenTM PE Rat Anti-Mouse CD209b (SIGN-R1)

Description

Multiparameter flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 mouse peritoneal exudate cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots mice, washed, and then stained with BD Pharmingen Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Rat IgM Isotype Control Cat. No. 553943; Left Plots or PE Rat Anti-Mouse CD209b antibody Cat. No. 568710/568711; Right Plots at 0.25 mug/test. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 mouse peritoneal exudate cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots mice, washed, and then stained with BD Pharmingen Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Rat IgM Isotype Control Cat. No. 553943; Left Plots or PE Rat Anti-Mouse CD209b antibody Cat. No. 568710/568711; Right Plots at 0.25 mug/test. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 mouse peritoneal exudate cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots mice, washed, and then stained with BD Pharmingen Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Rat IgM Isotype Control Cat. No. 553943; Left Plots or PE Rat Anti-Mouse CD209b antibody Cat. No. 568710/568711; Right Plots at 0.25 mug/test. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.