
559914 ·
BDB Bioscience · Cat: 559914
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes. Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes. Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes. Show More Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes. Show More Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes. Flow cytometric analysis of CD210 expression on mouse B220+ splenocytes. Mouse splenocytes were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; empty histograms or PE Rat Anti-Mouse CD210 Cat. No. 559914; filled histograms at 0.25 mug/test. Mouse splenocytes were preincubated on ice with recombinant mouse IL-10 0.25 mug, Cat. No. 550070 prior to staining with PE Rat Anti-Mouse CD210 Right Panel to determine the blocking effect of bound IL-10 protein on staining with conjugated antibody. Fluorescence histograms depicting CD210 or Ig isotype control expression were derived from gated events with the side and forward light-scattering characteristics of viable B220+ lymphocytes.