BD PharmingenTM PE Rat Anti-Mouse CD215 (IL-15Ralpha)

Description

Multicolor flow cytometric analysis of CD215 IL-15Ralpha expression on mature and immature bone marrow-derived dendritic cells. Mouse bone marrow-derived dendritic cells were either not stimulated Left Figures or stimulated Middle Figures by LPS overnight 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 561119 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Top Figures or PE Rat Anti-Mouse CD215 IL-15Ralpha antibody Cat. No. 568235; Bottom Figures at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL-15Ralpha or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative cells. Bone marrow-derived dendritic cells were identified as CD11c-positive cells. Histograms Right Figure were derived from gated cell subset show CD215 level for Isotype Control dashed line histogram and dendritic cells solid line histogram . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD215 IL-15Ralpha expression on mature and immature bone marrow-derived dendritic cells. Mouse bone marrow-derived dendritic cells were either not stimulated Left Figures or stimulated Middle Figures by LPS overnight 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 561119 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Top Figures or PE Rat Anti-Mouse CD215 IL-15Ralpha antibody Cat. No. 568235; Bottom Figures at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL-15Ralpha or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative cells. Bone marrow-derived dendritic cells were identified as CD11c-positive cells. Histograms Right Figure were derived from gated cell subset show CD215 level for Isotype Control dashed line histogram and dendritic cells solid line histogram . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD215 IL-15Ralpha expression on mature and immature bone marrow-derived dendritic cells. Mouse bone marrow-derived dendritic cells were either not stimulated Left Figures or stimulated Middle Figures by LPS overnight 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 561119 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Top Figures or PE Rat Anti-Mouse CD215 IL-15Ralpha antibody Cat. No. 568235; Bottom Figures at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL-15Ralpha or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative cells. Bone marrow-derived dendritic cells were identified as CD11c-positive cells. Histograms Right Figure were derived from gated cell subset show CD215 level for Isotype Control dashed line histogram and dendritic cells solid line histogram . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.