BD PharmingenTM PE Rat Anti-Mouse CD223
552380 ·
BDB Bioscience · Cat: 552380
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The expression of CD223 on activated T lymphocytes. C57BL/6 splenocytes were activated by culture for 2 days in the presence of immobilized anti-mouse CD3e mAb 145-2C11 Cat. No. 553057 and then were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061/553062 and either PE-conjugated rat IgG1, kappa isotype control mAb R3-34 Cat. No. 553925, left panel or PE-conjugated mAb C9B7W right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32, mAb 2.4G2 Cat. No. 553141/553142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.