BD PharmingenTM PE Rat Anti-Mouse CD226

Description

Multicolor flow cytometric analysis of CD226 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e Cat. No. 562600; Top Plots and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093; Bottom Plots antibodies, and either PE Rat IgG2b kappa Isotype Control Cat. No. 553989; Left Plots or PE Rat Anti-Mouse CD226 antibody Cat. No. 565663; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD3e or CD8a were derived from gated events with the forward and side light-scattering characteristics of viable 7-AAD-negative leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD226 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e Cat. No. 562600; Top Plots and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093; Bottom Plots antibodies, and either PE Rat IgG2b kappa Isotype Control Cat. No. 553989; Left Plots or PE Rat Anti-Mouse CD226 antibody Cat. No. 565663; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD3e or CD8a were derived from gated events with the forward and side light-scattering characteristics of viable 7-AAD-negative leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD226 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e Cat. No. 562600; Top Plots and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093; Bottom Plots antibodies, and either PE Rat IgG2b kappa Isotype Control Cat. No. 553989; Left Plots or PE Rat Anti-Mouse CD226 antibody Cat. No. 565663; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD3e or CD8a were derived from gated events with the forward and side light-scattering characteristics of viable 7-AAD-negative leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.