BD PharmingenTM PE Rat Anti-Mouse CD226 (DNAM-1)

Description

Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD226 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD226 DNAM-1 antibody Cat. No. 567357; Right Plot at 0.25 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD226 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific.