BD PharmingenTM PE Rat Anti-Mouse CD25

Description

Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were then stained with either PE Rat IgG2b, kappa Isotype Control dashed line histogram; Cat. No. 553989 or PE Rat Anti-Mouse CD25 antibody solid line histogram; Cat. No. 553075 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were then stained with either PE Rat IgG2b, kappa Isotype Control dashed line histogram; Cat. No. 553989 or PE Rat Anti-Mouse CD25 antibody solid line histogram; Cat. No. 553075 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were then stained with either PE Rat IgG2b, kappa Isotype Control dashed line histogram; Cat. No. 553989 or PE Rat Anti-Mouse CD25 antibody solid line histogram; Cat. No. 553075 . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.