BD PharmingenTM PE Rat Anti-Mouse CD273 (PD-L2)

Description

Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-Mouse CD273 Cat. No. 567217; Right Plot at 0.25mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.