BD PharmingenTM PE Rat Anti-Mouse CD278

Description

The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated top panels or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb anti-mouse CD3e, Cat. No. 553057, bottom panels . Both sets of cells were stained with either PE-conjugated rat IgG2b, kappa isotype control mAb A95-1 Cat. No. 553989, left panels or PE-conjugated mAb 7E.17G9 right panels , then FITC-conjugated mAb 53-6.7 anti-mouse CD8a, Cat. No. 553030/553031 . Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated top panels or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb anti-mouse CD3e, Cat. No. 553057, bottom panels . Both sets of cells were stained with either PE-conjugated rat IgG2b, kappa isotype control mAb A95-1 Cat. No. 553989, left panels or PE-conjugated mAb 7E.17G9 right panels , then FITC-conjugated mAb 53-6.7 anti-mouse CD8a, Cat. No. 553030/553031 . Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Show More The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated top panels or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb anti-mouse CD3e, Cat. No. 553057, bottom panels . Both sets of cells were stained with either PE-conjugated rat IgG2b, kappa isotype control mAb A95-1 Cat. No. 553989, left panels or PE-conjugated mAb 7E.17G9 right panels , then FITC-conjugated mAb 53-6.7 anti-mouse CD8a, Cat. No. 553030/553031 . Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA .