BD PharmingenTM PE Rat Anti-Mouse CD301a/b (MGL1/2)

Description

Multicolor flow cytometric analysis of CD301a/b MGL1/2 expression on mouse spleen or bone marrow-derived dendritic cells. Mouse splenocytes Left Plots and bone marrow-derived dendritic cells DCs Right Plots from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Top Plots or PE Rat Anti-Mouse CD301a/b MGL1/2 antibody Cat. No. 567050/567051; Bottom Plots at 1 mug/test. Spleen cells were also stained with FITC Hamster Anti-Mouse CD3 Cat. No. 553062 and FITC Rat Anti-Mouse CD19 Cat. No. 553785 antibodies. Bivariate pseudocolor density plots showing the correlated expression of CD301a/b MGL1/2 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable CD3- and CD19- splenocytes or bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD301a/b MGL1/2 expression on mouse spleen or bone marrow-derived dendritic cells. Mouse splenocytes Left Plots and bone marrow-derived dendritic cells DCs Right Plots from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Top Plots or PE Rat Anti-Mouse CD301a/b MGL1/2 antibody Cat. No. 567050/567051; Bottom Plots at 1 mug/test. Spleen cells were also stained with FITC Hamster Anti-Mouse CD3 Cat. No. 553062 and FITC Rat Anti-Mouse CD19 Cat. No. 553785 antibodies. Bivariate pseudocolor density plots showing the correlated expression of CD301a/b MGL1/2 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable CD3- and CD19- splenocytes or bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD301a/b MGL1/2 expression on mouse spleen or bone marrow-derived dendritic cells. Mouse splenocytes Left Plots and bone marrow-derived dendritic cells DCs Right Plots from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Top Plots or PE Rat Anti-Mouse CD301a/b MGL1/2 antibody Cat. No. 567050/567051; Bottom Plots at 1 mug/test. Spleen cells were also stained with FITC Hamster Anti-Mouse CD3 Cat. No. 553062 and FITC Rat Anti-Mouse CD19 Cat. No. 553785 antibodies. Bivariate pseudocolor density plots showing the correlated expression of CD301a/b MGL1/2 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable CD3- and CD19- splenocytes or bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.